He makes his first and last official appearance in Underworld: Evolution. Having just returned to the Sancta Helena after he and his squad had retrieved the bodies of Vampire Elders Viktor and Amelia, as well as the body of the Lycan leader Lucian, and possibly that of Vampire Death Dealer Rigel from their respective battles, he is next shown displaying video footage of each of the clean-up missions to Alexander who stands over him at a computer console on board the ship.
After police officers are engaged by Michael and Selene as well having seen Marcus and a deceased Lycan body, Alexander send Samuel and his team to silence them and destroy the Vampire safe house they had found, keeping the conflict a secret and the officers without proof of what they saw.
Samuel is then tasked to locate Selene and Michael after they escaped Marcus and bring them to Alexander.
After Marcus attacks the Sancta Helena, Samuel returns via helicopter with his platoon. As he arrives Samuel witness the entire crew slaughtered and a gravely wounded Alexander laying on the floor on top of his own blood. Samuel first reaction is to help his master, but Alexander refuses and asks to bring Selene to him. Samuel finds Selene in grief next to Michael's body, as she aggressively points her gun to Samuel, he claims that she will need Alexander's help against Marcus.
Samuel is seen putting Michael's body into a bag and placing it on board the helicopter. He then flies away with Selene and the surviving platoon, moments before the ship is detonated by Alexander himself who committed suicide in the process.
In William's Fortress, Samuel assists in the assault against an ancient beast, only to be attacked and turned moments later. The primitive lycanthrope virus resurrects him as a first-generation Werewolf along with the rest of his squad who, like him, fell victim to William. As he begun to transform, Selene hesitates a little before start shooting until she remembers what Andreas Tanis told her: that those turned by William are unable to take human form ever again after being infected by the virus. Samuel and the rest of the turned Cleaners are later killed by Selene with the help of the revived Michael.
- As the Commander of the Cleaners, Samuel's counterparts were Kahn (Death Dealers), Soren (Kraven's bodyguard) and Raze, (Lucian's enforcer).
- Main article: Samuel/Quotes